3FVN
Crystal structure of the human glutamate receptor, GluR5, ligand-binding core in complex with 9-deoxy-neodysiherbaine A in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-10-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.550, 50.814, 63.037 |
| Unit cell angles | 80.38, 84.37, 62.09 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.17251 |
| Rwork | 0.172 |
| R-free | 0.18966 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENATRY 2ZNT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.287 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 77805 | |
| <I/σ(I)> | 26 | 2.7 |
| Completeness [%] | 95.4 | 92 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 35% PEG3350, 0.3M LiSO4, 5mM 9-deoxy-neodysiherbaine, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
