3FUZ
Crystal structure of the human glutamate receptor, GluR5, ligand-binding core in complex with L-glutamate in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.617, 50.724, 63.254 |
| Unit cell angles | 93.52, 96.41, 117.86 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.19023 |
| Rwork | 0.189 |
| R-free | 0.21936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2znt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.313 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 59662 | |
| <I/σ(I)> | 19 | 2 |
| Completeness [%] | 96.3 | 91.3 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 35% PEG3350, 0.3M LiSO4, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
