3FUT
Apo-form of T. thermophilus 16S rRNA A1518 and A1519 methyltransferase (KsgA) in space group P21212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 137.046, 53.692, 69.354 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.040 - 1.520 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.334 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.039 | 0.250 |
| Number of reflections | 77536 | |
| <I/σ(I)> | 34.67 | 4.27 |
| Completeness [%] | 94.5 | 71.8 |
| Redundancy | 5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch technique under oil | 277 | 200 mM di-ammonium hydrogen phosphate and 20% w/v polyethylene glycole 3350, microbatch technique under oil, temperature 277K |
