3FTQ
Crystal structure of Septin 2 in complex with GppNHp and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97906 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.980, 118.440, 190.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.900 |
R-factor | 0.249 |
Rwork | 0.247 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qa5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.224 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.974 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.078 | 0.366 |
Number of reflections | 33762 | |
<I/σ(I)> | 4.81 | |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.2M Potassium Acetate, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |