3FTG
Crystal Structure of H2Db in complex with NP366-N3A variant peptide from influenza
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.640, 94.980, 132.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.229 |
| Rwork | 0.221 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cpl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.303 |
| Number of reflections | 18264 | 1905 |
| <I/σ(I)> | 27.55 | 6.4 |
| Completeness [%] | 98.7 | 97.7 |
| Redundancy | 7.22 | 6.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2M lithium sulfate, 28% PEG 3350, 0.1M sodium citrate, pH 5.6, vapor diffusion, hanging drop, temperature 293K |
