3FTF
Crystal structure of A. aeolicus KsgA in complex with RNA and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.130, 58.074, 68.981 |
Unit cell angles | 90.00, 109.82, 90.00 |
Refinement procedure
Resolution | 28.330 - 2.800 |
R-factor | 0.2411 |
Rwork | 0.239 |
R-free | 0.28795 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ftc |
RMSD bond length | 0.005 |
RMSD bond angle | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.880 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.144 | 0.397 |
Number of reflections | 6848 | |
<I/σ(I)> | 9 | 2.4 |
Completeness [%] | 84.2 | 49.9 |
Redundancy | 3.9 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 292 | 0.2 M KF, 40% MPD, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | KF | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 2 | KF | ||
4 | 1 | 2 | MPD |