3FTE
Crystal structure of A. aeolicus KsgA in complex with RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-07-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.173, 58.316, 91.118 |
Unit cell angles | 90.00, 107.37, 90.00 |
Refinement procedure
Resolution | 29.580 - 3.000 |
R-factor | 0.2353 |
Rwork | 0.233 |
R-free | 0.28069 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ftc |
RMSD bond length | 0.006 |
RMSD bond angle | 1.086 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.103 | 0.430 |
Number of reflections | 7560 | |
<I/σ(I)> | 13.4 | 1.9 |
Completeness [%] | 88.5 | 52.9 |
Redundancy | 5.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 292 | 0.2 M NH4NO3, 40% MPD, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NH4NO3 | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 2 | NH4NO3 | ||
4 | 1 | 2 | MPD |