3FSS

Structure of the tandem PH domains of Rtt106

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-17
Wavelength(s)	0.98089
Spacegroup name	C 1 2 1
Unit cell lengths	82.409, 92.206, 34.273
Unit cell angles	90.00, 105.16, 90.00

Refinement procedure

Resolution	29.508 - 1.432
R-factor	0.1818
Rwork	0.180
R-free	0.21020
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.508	1.463
High resolution limit [Å]	1.432	1.432
Number of reflections	45389
Completeness [%]	98.1	81
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	4% Tacsimate, 12% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K