3FRS
Structure of human IST1(NTD) (residues 1-189)(p43212)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-09-08 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.180, 57.180, 157.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.610 |
R-factor | 0.261 |
Rwork | 0.259 |
R-free | 0.29800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.177 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.13) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
High resolution limit [Å] | 2.610 | 5.620 | 2.610 |
Rmerge | 0.079 | 0.051 | 0.459 |
Number of reflections | 8387 | ||
<I/σ(I)> | 13.47 | ||
Completeness [%] | 97.7 | 93.6 | 88.3 |
Redundancy | 3.8 | 3.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | 10mM Tris-HCL, pH 8.0, 350mM NaCl, 1mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | 10mM Tris-HCL, pH 8.0, 350mM NaCl, 1mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 296K |