3FRO
Crystal structure of Pyrococcus abyssi glycogen synthase with open and closed conformations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.998, 139.913, 159.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.910 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25500 |
| Starting model (for MR) | 2bis |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.825 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | 0.410 |
| Number of reflections | 58364 | |
| <I/σ(I)> | 8.4 | 1.1 |
| Completeness [%] | 99.7 | 97.6 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 26-30% MPD, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
