3FRI
Structure of the 16S rRNA methylase RmtB, I222
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2007-05-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.480, 94.460, 98.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.550 - 1.800 |
| R-factor | 0.2125 |
| Rwork | 0.212 |
| R-free | 0.23290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b89 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.375 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.394 |
| Number of reflections | 24613 | |
| <I/σ(I)> | 17.33 | 3.21 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 3.58 | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG 3350, 50mM Bis-Tris pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
