3FRH
Structure of the 16S rRNA methylase RmtB, P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2007-12-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.856, 48.304, 54.558 |
Unit cell angles | 90.00, 115.05, 90.00 |
Refinement procedure
Resolution | 25.400 - 1.200 |
R-factor | 0.1388 |
Rwork | 0.139 |
R-free | 0.15930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b89 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.952 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.078 | 1.400 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.039 | 0.130 |
Number of reflections | 71817 | |
<I/σ(I)> | 14.56 | 7.73 |
Completeness [%] | 95.6 | 94.3 |
Redundancy | 2.2 | 2.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG 3350, 50mM bis-tris pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |