3FQD

Crystal Structure of the S. pombe Rat1-Rai1 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-08
Detector	Mar m-165 CCD
Wavelength(s)	0.9806
Spacegroup name	P 21 21 2
Unit cell lengths	96.690, 190.869, 84.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.22
Rwork	0.218
R-free	0.25800
RMSD bond length	0.008
RMSD bond angle	1.019
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.073	0.040	0.356
Number of reflections	77326
<I/σ(I)>	17.502
Completeness [%]	92.5	99.2	59.9
Redundancy	4	4.3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	0.3 M sodium malonate (pH 5.0), 10 mM DTT and 14 % (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K