3FQ8
M248I mutant of GSAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Wavelength(s) | 1.1154 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.680, 106.951, 122.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.900 - 2.000 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hp2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 56587 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 273 | pH 6.8, VAPOR DIFFUSION, temperature 273K |
