3FPN

Crystal structure of UvrA-UvrB interaction domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Collection date	2008-10-08
Wavelength(s)	0.97949
Spacegroup name	P 41 21 2
Unit cell lengths	84.491, 84.491, 60.874
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.390 - 1.800
R-factor	0.231
Rwork	0.230
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2R6F(residues 131-153 and 200-245 chain A) PDB ENTRY 1T5L (residues 157-250 chain A)
RMSD bond length	0.012
RMSD bond angle	1.367
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.043	0.023	0.443
Number of reflections	20904
<I/σ(I)>	70.679
Completeness [%]	99.9	98.4	100
Redundancy	14.2	12.7	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop vapor diffusion	5	295	PEG4000, NaCl, sodium acetate, Tris-HCl, pH 5.0, hanging drop vapor diffusion, temperature 295K