3FPB
The Structure of Sarcoplasmic Reticulum Ca2+-ATPase Bound To Cyclopiazonic acid with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.115872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 175.360, 69.870, 143.500 |
Unit cell angles | 90.00, 107.16, 90.00 |
Refinement procedure
Resolution | 24.942 - 2.550 |
R-factor | 0.185 |
Rwork | 0.183 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o9j |
RMSD bond length | 0.029 |
RMSD bond angle | 2.430 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.600 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 54351 | |
<I/σ(I)> | 1.8 | |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 284 | 8% PEG 3350, 21% GLYCEROL, 20mM MGCL2, 0.1mM EGTA, 20mM MES, pH 6.10, VAPOR DIFFUSION, HANGING DROP, temperature 284K |