3FP2

Crystal structure of Tom71 complexed with Hsp82 C-terminal fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	200
Detector technology	CCD
Collection date	2008-10-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	47.863, 116.288, 150.743
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.990 - 1.980
R-factor	0.20107
Rwork	0.199
R-free	0.23645
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fp3
RMSD bond length	0.018
RMSD bond angle	1.524
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	7.990
High resolution limit [Å]	1.980	1.980
Number of reflections	53827
Completeness [%]	1.0	0.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG6K, Ethylene Glyco 5%, NaCl 0.15M, Tris 10mM, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K