3FOC
Tryptophanyl-tRNA synthetase from Giardia lamblia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.714, 140.147, 90.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.090 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ip1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.003 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 4.520 | 2.090 |
| Rmerge | 0.089 | 0.051 | 0.610 |
| Number of reflections | 77780 | ||
| <I/σ(I)> | 20.921 | ||
| Completeness [%] | 97.3 | 99.9 | 82 |
| Redundancy | 6.9 | 7.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.2 | 298 | 2.7 M (NH4)2SO4, 0.1 M Citric acid pH 5.2, 5mM DTT, VAPOR DIFFUSION, temperature 298K |
