3FNJ

Crystal structure of the full-length lp_1913 protein from Lactobacillus plantarum, Northeast Structural Genomics Consortium Target LpR140

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97905
Spacegroup name	P 43
Unit cell lengths	119.365, 119.365, 67.791
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.700
R-factor	0.231
Rwork	0.231
R-free	0.27600
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.2 & XtalView)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.085	0.421
Number of reflections	51464
<I/σ(I)>	26.7	4.4
Completeness [%]	99.6	100
Redundancy	7.5	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch under oil	7	293	Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 100 mM Bis-Tris Propane (pH 7.0) and 1880 mM sodium thiosulfate. , Microbatch under oil, temperature 293K