3FN2
Crystal structure of a putative sensor histidine kinase domain from Clostridium symbiosum ATCC 14940
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-09 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97945, 0.97921 |
| Spacegroup name | H 3 |
| Unit cell lengths | 107.252, 107.252, 49.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.475 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.091 | 0.486 |
| Number of reflections | 16144 | |
| <I/σ(I)> | 30.418 | |
| Completeness [%] | 95.7 | 73.6 |
| Redundancy | 5.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 296 | 0.1M Acetate pH 4.5, 0.8M NaH2PO4, 1.4M K2HPO3, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
