3FMT
Crystal structure of SeqA bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 121.839, 121.839, 279.133 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.172 - 2.983 |
| R-factor | 0.2194 |
| Rwork | 0.218 |
| R-free | 0.24480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1LRR and 1XRX |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.877 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.170 | 3.110 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.088 | 0.527 |
| Number of reflections | 30745 | |
| <I/σ(I)> | 13.5 | 1.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 18% MPD, 0.4 M ammonium acetate, 0.1 M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | ammonium acetate | ||
| 3 | 1 | 1 | sodium citrate | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | ammonium acetate | ||
| 6 | 1 | 2 | sodium citrate |
