3FM7
Quaternary Structure of Drosophila melanogaster IC/Tctex-1/LC8; Allosteric Interactions of Dynein Light Chains with Dynein Intermediate Chain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | .98 |
Spacegroup name | P 62 |
Unit cell lengths | 115.790, 115.790, 90.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 100.000 - 3.500 |
R-factor | 0.18001 |
Rwork | 0.170 |
R-free | 0.27470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | chains A and F in pdb structure 2PG1 |
RMSD bond length | 0.000 |
RMSD bond angle | 0.047 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.640 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.088 | 0.358 |
Number of reflections | 8745 | |
<I/σ(I)> | 15.9 | 6.2 |
Completeness [%] | 98.8 | 100 |
Redundancy | 7.1 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Hanging Drop | 6.5 | 277.15 | 16% PEG 8K, 100 mM sodium cacodylate, 200 mM calcium acetate, pH 6.5, Hanging Drop, temperature 277.15K |