3FLP
Crystal structure of native heptameric SAP-like pentraxin from Limulus polyphemus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-06 |
| Detector | ADSC |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.320, 167.560, 140.870 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 45.270 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.201 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Dimer created from A subunit of native octameric Limulus SAP |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.1.4) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.270 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.167 |
| Total number of observations | 54093 | |
| Number of reflections | 180419 | |
| <I/σ(I)> | 5.435 | 3.9 |
| Completeness [%] | 89.7 | 93.2 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | PEG 6000, CaCl2, MES, pH 7.4, sitting drop, temperature 298.0K, VAPOR DIFFUSION, SITTING DROP |
