3FLH
Crystal structure of lp_1913 protein from Lactobacillus plantarum,Northeast Structural Genomics Consortium Target LpR140B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97892 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.892, 47.429, 62.769 |
Unit cell angles | 90.00, 113.55, 90.00 |
Refinement procedure
Resolution | 19.880 - 2.000 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.24800 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELX (then SOLVE/RESOLVE) |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.103 | 0.371 |
Number of reflections | 41366 | |
<I/σ(I)> | 16.52 | 4.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 293 | Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 100 mM sodium acetate (pH 5.0), 40% PEG 4k, and 100 mM sodium bromide. Microbatch under oil, temperature 293K |