3FLD
Crystal structure of the trai c-terminal domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97835, 0.97873, 0.97126 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.800, 139.751, 126.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 24509 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 89.4 | 89.4 |
| Redundancy | 6.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1 M ammonium sulfate, 0.1 M MES, pH 6.0, cryoprotected in 1.8 M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
