3FJO

Structure of chimeric YH CPR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	146.800, 60.700, 78.860
Unit cell angles	90.00, 103.36, 90.00

Refinement procedure

Resolution	19.860 - 2.500
R-factor	0.22549
Rwork	0.222
R-free	0.28994
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1AMO (FAD domain) 2BF4 (FMN domain)
RMSD bond length	0.008
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	23129
<I/σ(I)>	12.7	4.2
Completeness [%]	97.4	94.9
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEGII from Nextal condition G11, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K