3FJK
Crystal structure of A66C mutant of Human acidic fibroblast growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-23 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.356, 110.799, 67.326 |
| Unit cell angles | 90.00, 105.30, 90.00 |
Refinement procedure
| Resolution | 28.700 - 2.150 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.096 | 0.380 |
| Number of reflections | 33702 | |
| <I/σ(I)> | 3.13 | |
| Completeness [%] | 85.7 | 100 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.3 M Na formate, 0.15M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
