3FJI
Crystal structure of K12V/C83I/C117V mutant of Human acidic fibroblast growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-03-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.750, 108.076, 67.675 |
| Unit cell angles | 90.00, 103.73, 90.00 |
Refinement procedure
| Resolution | 29.090 - 2.550 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.090 | 2.610 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.095 | 0.256 |
| Number of reflections | 19041 | |
| <I/σ(I)> | 2.98 | |
| Completeness [%] | 87.5 | 94.8 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.0M Na formate, 0.1M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
