3FJ9
Crystal structure of F85W mutant of Human acidic fibroblast growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-23 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5481 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.232, 95.771, 109.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.860 - 1.900 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.860 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.379 |
| Number of reflections | 27769 | |
| <I/σ(I)> | 3.64 | |
| Completeness [%] | 90.3 | 89.2 |
| Redundancy | 7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3M Na-formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
