3FJ5
Crystal structure of the c-src-SH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-03-03 |
Detector | Mar CCD-165 |
Wavelength(s) | 0.97 |
Spacegroup name | P 65 |
Unit cell lengths | 46.596, 46.596, 128.064 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.650 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hda |
RMSD bond length | 0.010 |
RMSD bond angle | 2.029 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.357 | 40.360 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.078 | 0.064 | 0.543 |
Total number of observations | 3858 | 20221 | |
Number of reflections | 19644 | ||
<I/σ(I)> | 4.701 | 8.8 | 1.4 |
Completeness [%] | 95.6 | 90.3 | 95 |
Redundancy | 7.1 | 6.6 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 288 | 1.7M Ammonium sulphate, 5% PEG300, 10% glycerol, 0.1M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 288K |