3FIU

Structure of NMN synthetase from Francisella tularensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.432, 126.438, 152.531
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.850
R-factor	0.18662
Rwork	0.184
R-free	0.23338
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kqp
RMSD bond length	0.014
RMSD bond angle	1.391
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Number of reflections	79468
Completeness [%]	94.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M K/Na tartrate, 16% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K