3FIP
Crystal structure of Usher PapC translocation pore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2008-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 120.036, 120.036, 354.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.679 - 3.154 |
| R-factor | 0.289 |
| Rwork | 0.285 |
| R-free | 0.35900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 41032 | |
| <I/σ(I)> | 34.8 | 2.4 |
| Completeness [%] | 96.3 | |
| Redundancy | 6.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.9 | 293 | 3.3M NaCl, 0.06%LDAO, 4% 2,5 hexanidiol, 20mM Tris, pH 4.9, EVAPORATION, temperature 293K |
