3FIJ
Crystal structure of a uncharacterized protein lin1909
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.056, 131.205, 93.290 |
| Unit cell angles | 90.00, 108.79, 90.00 |
Refinement procedure
| Resolution | 43.100 - 2.300 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.656 |
| Number of reflections | 91961 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M MES 6.5, 12% PEG 20,000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
