3FI7
Crystal Structure of the autolysin Auto (Lmo1076) from Listeria monocytogenes, catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 133.500, 133.500, 88.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.480 - 2.350 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.883 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 19770 | |
| <I/σ(I)> | 37.71 | 6.49 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 15.9 | 16.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1M ammonium sulfate, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
