3FI0
Crystal Structure Analysis of B. stearothermophilus Tryptophanyl-tRNA Synthetase Complexed with Tryptophan, AMP, and Inorganic Phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-09-15 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97934,0.97945,0.97117 |
Spacegroup name | P 1 |
Unit cell lengths | 122.032, 122.396, 122.341 |
Unit cell angles | 79.90, 80.52, 79.81 |
Refinement procedure
Resolution | 25.000 - 2.700 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.22000 |
RMSD bond length | 0.044 |
RMSD bond angle | 3.380 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.069 |
Number of reflections | 203226 |
Completeness [%] | 97.7 |
Redundancy | 3.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.58 | 315 | 2.18 M K2HPO4, 0.6% (v/v) PEG 400, pH 6.58, dialysis, temperature 315K |