3FHU

Crystal structure of type IV b pilin from Salmonella typhi

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-09
Detector	Brandeis B4
Wavelength(s)	0.97939, 0.97978, 0.95
Spacegroup name	P 21 21 2
Unit cell lengths	77.880, 114.530, 31.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
Rwork	0.220
R-free	0.26000
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	21898
<I/σ(I)>	26.5	7.39
Completeness [%]	95.8
Redundancy	4.3	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	4M Sodium Formate, VAPOR DIFFUSION, SITTING DROP, temperature 294K