3FHJ
Independent saturation of three TrpRS subsites generates a partially-assembled state similar to those observed in molecular simulations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 223.606, 91.989, 158.322 |
| Unit cell angles | 90.00, 134.01, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.650 |
| R-factor | 0.21 |
| Rwork | 0.200 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fi0 |
| RMSD bond length | 0.039 |
| RMSD bond angle | 3.132 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.850 |
| High resolution limit [Å] | 2.500 | 2.650 |
| Rmerge | 0.081 | 0.414 |
| Number of reflections | 161648 | |
| <I/σ(I)> | 4.22 | |
| Completeness [%] | 97.6 | 77.5 |
| Redundancy | 3.48 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.58 | 315 | 2.28M K2HPO4, 0.6% (v/v) PEG 400, pH 6.58, dialysis, temperature 315K |
