3FHC
Crystal structure of human Dbp5 in complex with Nup214
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99988 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 117.409, 134.707, 152.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.650 - 2.800 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2OIT and 3FHT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.138 | 0.364 |
| Number of reflections | 30169 | |
| <I/σ(I)> | 11.59 | 3.31 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.13 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 50mM MES pH 6.0, 900mM Na citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
