3FFZ
Domain organization in Clostridium butulinum neurotoxin type E is unique: Its implication in faster translocation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-10 |
| Detector | BRANDEIS 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.430, 172.570, 137.265 |
| Unit cell angles | 90.00, 99.84, 90.00 |
Refinement procedure
| Resolution | 29.490 - 2.650 |
| R-factor | 0.253 |
| Rwork | 0.253 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1EPW 3bta |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.106 | 0.278 |
| Number of reflections | 98598 | |
| <I/σ(I)> | 9 | 1.5 |
| Completeness [%] | 89.5 | 48 |
| Redundancy | 6.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG 8000, 0.1M NaCl, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
