3FFU

Structure of the RNA pyrophosphohydrolase BdRppH in complex with GTP and magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-10-10
Detector	RIGAKU RAXIS4
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	68.732, 68.749, 93.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.610 - 2.800
R-factor	0.229
Rwork	0.226
R-free	0.30100
RMSD bond length	0.010
RMSD bond angle	1.393
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.054	0.030	0.714
Number of reflections	11207
<I/σ(I)>	31.443
Completeness [%]	98.5	93.8	99.8
Redundancy	3.8	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7	298	PEG 4000, Na acetate, pH 7, hanging drop, temperature 298K