3FFO

F17b-G lectin domain with bound GlcNAc(beta1-2)man

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-05-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.8453
Spacegroup name	P 62
Unit cell lengths	87.389, 87.389, 56.809
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.920 - 2.100
R-factor	0.237
Rwork	0.233
R-free	0.27100
Starting model (for MR)	1o9v
RMSD bond length	0.005
RMSD bond angle	0.970
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.065	0.340
Number of reflections	14544
<I/σ(I)>	21	6.6
Completeness [%]	99.8	100
Redundancy	10.8	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	1.2M lithium sulphate, 10mM nickel chloride, 100mM TRIS, pH 8.5, VAPOR DIFFUSION, temperature 293K