3FFB

Gi-alpha-1 mutant in GDP bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	298
Detector technology	CCD
Collection date	2006-11-06
Detector	ADSC QUANTUM 315
Spacegroup name	I 4
Unit cell lengths	121.761, 121.761, 68.714
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.800 - 2.570
Rwork	0.181
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bof
RMSD bond length	0.012
RMSD bond angle	1.372
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.800	2.700
High resolution limit [Å]	2.570	2.570
Rmerge	0.060	0.275
Number of reflections	16157
<I/σ(I)>		7.4
Completeness [%]	100.0	100
Redundancy	7.3	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	100 mM Sodium Acetate pH 5.9-6.3, 1.8-2.1 M Ammonium Sulphate, VAPOR DIFFUSION, temperature 293K, pH 6.0