3FF5
Crystal structure of the conserved N-terminal domain of the peroxisomal matrix-protein-import receptor, Pex14p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.8 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 90.570, 90.570, 90.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.18104 |
| Rwork | 0.179 |
| R-free | 0.21081 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.023 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.076 | 0.361 |
| Number of reflections | 11491 | |
| <I/σ(I)> | 39.6 | 6.3 |
| Completeness [%] | 100.0 | 93.2 |
| Redundancy | 17.8 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2M sodium citrate, 100mM HEPES(pH7.5), 10%(v/v) glycerol, 0.2%(w/v) n-decylphosphocholine, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
