3FEV
Crystal structure of the chimeric muscarinic toxin MT7 with loop 1 from MT1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.007 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.140, 71.975, 89.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.300 |
| R-factor | 0.2156 |
| Rwork | 0.215 |
| R-free | 0.23322 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vlw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.367 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.040 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.155 | 0.650 |
| Number of reflections | 39130 | |
| <I/σ(I)> | 3 | 1.4 |
| Completeness [%] | 88.5 | 71.6 |
| Redundancy | 5.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 50mM sodium acetate, 0.02% NaN3, 1M ammonium sulfate, 90mM sodium citrate, 8% 1,6 hexanediol, 6% propanol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
