3FEQ
Crystal structure of uncharacterized protein eah89906
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-02 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 |
Unit cell lengths | 113.208, 108.048, 171.132 |
Unit cell angles | 81.75, 80.36, 74.40 |
Refinement procedure
Resolution | 20.000 - 2.630 |
R-factor | 0.23902 |
Rwork | 0.238 |
R-free | 0.28148 |
Structure solution method | MR |
Starting model (for MR) | 2R8C |
RMSD bond length | 0.009 |
RMSD bond angle | 1.080 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.610 | 2.610 |
Rmerge | 0.206 | 0.760 |
Number of reflections | 227280 | |
<I/σ(I)> | 3.2 | 1 |
Completeness [%] | 98.0 | 97.1 |
Redundancy | 3.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 6% PEG 8000, MES, PH 6.0, 200MM SODIU ACYTATE,10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |