3FEH
Crystal structure of full length centaurin alpha-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.96863 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.055, 52.380, 65.023 |
| Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
| Resolution | 37.010 - 1.900 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.474 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.11) |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.083 | 0.056 | 0.377 |
| Number of reflections | 27553 | ||
| Completeness [%] | 91.1 | 95.8 | 53.8 |
| Redundancy | 3.1 | 3.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% PEG3350, 0.2M ammonium sulfate, 0.1M Bis-Tris, pH 6.5, vapor diffusion, sitting drop, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% PEG3350, 0.2M ammonium sulfate, 0.1M Bis-Tris, pH 6.5, vapor diffusion, sitting drop, temperature 291K |
