3FDZ
Crystal structure of phosphoglyceromutase from burkholderia pseudomallei 1710b with bound 2,3-diphosphoglyceric acid and 3-phosphoglyceric acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-30 |
Detector | SATURN |
Spacegroup name | P 1 |
Unit cell lengths | 44.390, 48.480, 62.090 |
Unit cell angles | 106.03, 91.54, 107.50 |
Refinement procedure
Resolution | 19.750 - 2.250 |
R-factor | 0.187 |
Rwork | 0.183 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.526 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.310 | |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.071 | 0.345 |
Number of reflections | 20934 | |
<I/σ(I)> | 11.43 | 2.6 |
Completeness [%] | 93.4 | 90.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 289 | 30% PEG 600, 5% PEG 1000, 10% GLYCEROL, 100 MM MES PH 6.0, CRYSTALS SOAKED OVERNIGHT WITH 20 MM 3-PHOSPHOGLYCERIC ACID, VAPOR DIFFUSION, VAPOR DIFFUSION, SITTING DROP, temperature 289K |