3FDS
Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.986, 86.414, 97.582 |
| Unit cell angles | 90.00, 107.30, 90.00 |
Refinement procedure
| Resolution | 29.880 - 2.050 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1jx4 and 2ijx |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.861 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.073 | 0.519 |
| Number of reflections | 64500 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.5 | 89.7 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10% PEG3350, 125mM LiCl, pH 7.5, vapor diffusion, hanging drop, temperature 298K |
