3FDL
Bim BH3 peptide in complex with Bcl-xL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.111, 35.684, 62.760 |
| Unit cell angles | 90.00, 111.54, 90.00 |
Refinement procedure
| Resolution | 29.190 - 1.780 |
| R-factor | 0.16295 |
| Rwork | 0.160 |
| R-free | 0.20769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2P1L without Beclin BH3 ligand |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.046 | 0.163 |
| Number of reflections | 14264 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 96.0 | 94.9 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1M Sodium citrate, 0.1M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
