3FDG
The crystal structure of the dipeptidase AC, Metallo peptidase. MEROPS family M19
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.246, 50.683, 81.594 |
| Unit cell angles | 82.42, 73.61, 68.18 |
Refinement procedure
| Resolution | 78.330 - 1.800 |
| R-factor | 0.17779 |
| Rwork | 0.175 |
| R-free | 0.22260 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.837 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.330 | 1.848 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.093 | 0.270 |
| Number of reflections | 52476 | |
| <I/σ(I)> | 16.48 | 2.44 |
| Completeness [%] | 90.7 | 55.18 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M Magnesium chloride hexahydrate, 0.1M Tris, 25% w/v Polyethylene glycol 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
